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Automated 2- step purification of mouse antibody IgG1

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Agregado hace por MetrohmHispania
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Dr. Yannick Krauke from the KNAUER lab describes an automated purification of mouse immunoglobulin antibodies (IgG) from cell culture. The cell culture was applied with a feeding pump on a proteinA affinity column to capture and purify the antibodies. These were kept in the system and in a second step applied on a gel filtration column for buffer exchange. The obtained antibody was now dissolved in the desired storage and/or working buffer. This application note highlights the possibility of automated purification with the AZURA BIO LC system without manual interaction during purification process. If you want to read the complete application note, check www.knauer.net/prod/vbs0063
or learn more on www.knauer.net

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Based in Berlin, Germany, KNAUER has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including Ultra-high performance liquid chromatography (UHPLC), Analytical & Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-Column Chromatography / Simulated moving bed (SMB), Sample preparation, Dosing / Metering / Pumping, Detection, Osmometry, OEM solutions, and more.

Website: http://www.knauer.net/

Analytical HPLC / UHPLC: http://www.knauer.net/en/systems-solutions/analytical-hplc-uhplc.html
Preparative HPLC: http://www.knauer.net/systems-solutions/preparative-hplc.html
FPLC: http://www.knauer.net/en/systems-solutions/fplc.html
Multi-Column Chromatography / SMB: http://www.knauer.net/en/systems-solutions/multicolumn-applications-smb/smb.html
Sample preparation: http://www.knauer.net/en/systems-solutions/sample-preparation.html
Dosing, metering, & pumping: http://www.knauer.net/systems-solutions/dosing-metering-pumping.html
Detection: http://www.knauer.net/en/products/detectors-flow-cells.html
Osmometry: http://www.knauer.net/product/k_7400s_semi_micro_osmometer.html

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